Antimicrobial activity of different extracts of pongamia pinnata p. Micropropagation and mediumterm conservation of rosa. Plant details information about pongamia pinnata plant. Pongamia or pongamia pinnata is an average sized tree. The parenchymatous cell that associated with the fibers was more thick walled and contained bigger clustertype calcium oxalate crystals. Morphological and molecular level of genetic diversity among pongamia pongamia pinnata l. Application of tissue culture techniques for genetic up gradation of economically important plants has been reported. Principles of tissue cult ure and micropropagation. Pharmacognostic and phytochemical investigation of pongamia. Evaluation of antibacterial activity ofpongamia pinnata l. A complete protocol is presented for the first time for the micropropagation of pongamia pinnata, a biofuel tree, using cotyledonary nodes derived from axenic seedlings. Pierre accessions s p ahlawat1, r v kumar 2, rajeev ranjan 1, s k pandey, d c joshi and s k dhyani 1icarcentral agroforestry research institute, jhansi 284 002, india 2icarindian grassland and fodder research institute, jhansi 284 003. Pongamia pinnata click image to enlarge photographs by. Pdf micropropagation of mature pongamia pinnata pierre.
Pdf merge combinejoin pdf files online for free soda pdf. Soda pdf merge tool allows you to combine two or more documents into a single pdf file for free. Karanja cake activated carbon as an adsorbent is that it facilitates complete utilisation of pongamia pinnata besides contributing to waste management. Micropropagation of mature pongamia pinnata pierre springerlink. Tissue culture is the in vitro aseptic culture of cells, tissues, organs or whole plant. It is the fact that micropropagation is the only commercially viable method of clonal propagation of most of the horticultural crops. Biotechnology for micropropagation and enhancing variations. Plants can be propagated by sexual through generation of seeds or asexual through multiplication of vegetative parts means. Michael kane environmental horticulture department university of florida rapid clonal in vitro propagation of plants from cells, tissues or organs cultured aseptically on defined media contained in culture vessels maintained under controlled conditions of light and temperature micropropagation in vitro propagation. The study showed that in general, using activated carbons for biodiesel purification resulted in higher yields and better fuel properties. Reactiveextraction of pongamia seeds for biodiesel production. Biochemical activities in different accession of pongamia. Micropropagation is the asexual propagation of plants from growing tissue be it whole. The present paper deals with chemical examination of defatted seeds of the plant pongamia pinnata l.
Pongamia pinnata is a legume plant which has great potential to be used as a biofuel feedstock. List of various diseases cured by pongamia pinnata. Analysis of genetic diversity in pongamiapongamia pinnata l. A protocol for in wk propagation of this plant was standardized using seedling explants. This research work consists protein profiling and enzyme estimation of p. In pongamia, no such undulations were noted in the axillary meristems after 4 wk of culture on tdz, but the swelling in the meristem was obvious. Pongamia pinnata pierre is a tree legume, having potential in production of raw material for biodiesel. The pongamia seeds were crushed in retsch model sm100 cutting mill hann, germany with feed rate up to 10kgh, feed size of pongamia pinnata l. Pongamia is a medium size, nitrogen fixing tree native to india, indonesia, malaysia, and myanmar.
Antimicrobial activity of different extracts of pongamia pinnata. Peter 4 1department of botany, providence womens college, calicut 673009, kerala, india 2 project coordinator, all india coordinated research project on spices, indian institute of. Use of the wood is limited to cabinetmaking, cartwheels. Pdf pongamia pinnata is gaining lot of importance as a potential oil yielding tree. Pongamia pinnata, floral phenology, pod maturation, fruit colours, seed and seedling quality characters. Incorporation of ba, kn, or z was ineffective in enhancing sprouting frequency or. However, further study on the phytochemistry and the mechanisms of the chemical constituents in exhibiting certain biological activities are required to fully understand the phytochemical profile and the complex pharmacological effects. Chapter2 micropropagation and large scale culture of c. Micropropagationomicsgroupjournal of biotechnology and. Read this article to learn about the techniques, factors, applications and disadvantages of micro propagation process and also about production of diseasefree plants and its merits and demerits. Studies and research in applied zoology, school of biological sciences, kuvempu university, karnataka, india.
Please visit our seed store to view current selections. Pdf clonal propagation of pongamia pinnata pierre through. Fruit is brown in color and seed pods are poisonous. Hplc analysis was performed on a c 18 column using an 85. Micropropagation definition of micropropagation at. Evaluation of antibacterial activity of pongamia pinnata l. Which has been found to consist of five sugars viz dmannose, dglucose, dxylose, raffinose, lfructose along with five. Sprouting of buds was 64% on medium devoid of plant growth regulators pgr. Micropropagation definition, the propagation of plants from tissue cultures, often from single cells. Uses, benefits, cures, side effects, nutrients in pongamia pinnata. May 24, 2012 biochemical activities in different accession of pongamia pinnata l. Pongamia millettia pinnata, formerly known as pongamia pinnata, is a treeshrub. A rapid, simple and specific reversedphase hplc method has been developed for analysis of karanjin in pongamia pinnata linn.
Peter 4 1department of botany, providence womens college, calicut 673009, kerala, india 2 project coordinator, all india coordinated research. It is essential that the surface of the explant is sterilised to remove all microbial contamination. The pongamia seeds were crushed in retsch model sm100 cutting mill hann, germany with feed rate up to 10kgh, feed size of of 0. Micropropagation and mediumterm conservation of rosa pulverulenta somayeh kavand, maryam jafarkhani kermani, ali haghnazari, pegah khosravi and mohamad reza azimi tissue culture and gene transformation department, agricultural biotechnology research institute of iran,islamic republic of iran. Pappilonaceae is a nonedible oil of indian origin lakshmikanthan, 1978. Find highquality stock photos that you wont find anywhere else. How to merge pdfs and combine pdf files adobe acrobat dc. Aug 18, 2007 murashige and skoogs ms basal medium with benzylaminopurine ba, kinetin kn, zeatin z, and thidiazuron tdz were tested for induction of multiple shoots from maturetreederived axillary meristems of pongamia pinnata. Plant tissue culture offers new ways for the improvement of this crop after many years of recalcitrance. Biotechnology for micropropagation and enhancing variations in vanilla minoo divakaran 1, k. Pongamia pinnata a sustainable biofuel plant species for semi arid climatic. Multiplication of genetically identical copies of a cultivar by asexual reproduction is called clonal propagation.
It even has the potential to expand itself in the sun protected areas as well. Plant tissue culture case study 3 demonstration of. Ps2pdf free online pdf merger allows faster merging of pdf files without a limit or watermark. It is antihelmintic and useful in beriberi, ophthalmology, dermatopathy, vaginopathy, and ulcers. Combine pdfs in the order you want with the easiest pdf merger available. Due to recent interest in biofuels, pongamia is often the generic name used when referring to millettia pinnata, a tree being explored for producing biodiesel.
These crushed seeds were separated by sieves to give. Plant cell division is slow compared to the growth of bacteria and fungi, and even minor contaminants will easily overgrow the plant tissue culture. Macropropagation of acacias authorstream presentation. Easily combine multiple files into one pdf document. Explants carrying performed vegetative bud suitable for enhanced axillary branching. Pierre leguminaceae, subfamily papilionoideae is a medium sized tree commonly called as karanja that generally attains a height of about 1020 m and a trunk diameter of more than 50 cm pongamia. Medicinal uses, phytochemistry and pharmacology of pongamia.
Micropropagation of pongamia pinnata through enhanced. Medium sized tree growing up to 40 ft with a large spreading canopy up to 40 ft. Jain 1, shashi alok 1, deepak kailasiya 1, vinod kr. It is often known by the synonym pongamia pinnata as it was moved to the genus millettia only recently. S department of agriculture was given seeds from sri lanka. Abstractbibliographic data and the results of present domestic experiments show that paulownia species can be grown easily, have high biomass production, favourable energetic parameters and modest requirements as to site quality. Preparation of biodiesel from crude oil of pongamia pinnata. It has been successfully introduced to humid tropic lowland. A brownish green or bluish black coloration that resulting with the addition of few drops 0. Deze gratis online tool maakt het mogelijk om meerdere pdf bestanden of afbeeldingen te combineren in een pdf document. The fresh bark of pongamia pinnata is sweet and mucilaginous to taste, soon become bitter and acrid.
Pierre accessions s p ahlawat1, r v kumar 2, rajeev ranjan 1, s k pandey, d c joshi and s k dhyani 1icarcentral agroforestry research institute, jhansi 284 002, india 2icarindian grassland and fodder research institute, jhansi 284 003, india. Names of pongamia pinnata in various languages of the world are also given. Development and validation of an hplc method for karanjin in. Native to tropical and subtropical areas, the pongam tree, or pongamia pinnata, was first introduced to hawaii in the 1860s.
Development and validation of an hplc method for karanjin. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. New tree species for agroforestry and energy purposes. Preparation of biodiesel from crude oil of pongamia. An efficient protocol for micropropagation and genetic stability analysis of melia dubia cav. The place having rainfall of 5002500 cm together with a temperature range of 1040 degree celsius is suitable for growing pongamia trees. The fibers and sclereids of the pedicel were similar to those found in the pods but were larger. Aug 23, 20 the legume tree pongamia pinnata also called millettia pinnata is gaining importance as a biofuel feedstock tree because of the abundant annual production of oilrich seeds, adaptation to a wide range of geoclimatic conditions and significant resistance to abiotic stress, such as waterdeficit, salinity and acidity of soils. How pongamia pinnata is effective for various diseases is listed in repertory format. Antimicrobial efficacy of some medicinal plant extract. This free online tool allows to combine multiple pdf or image files into a single pdf document. Our pdf merger allows you to quickly combine multiple pdf files into one single pdf document, in just a few clicks.
Millettia pinnata is a species of tree in the pea family, fabaceae, native to eastern and tropical asia, australia and pacific islands. Successfully been grown and conserved by micropropagation because of. Autotrophic plants, green algae, and red algae which generally acquire nutrients through photosynthesis and display alternation of generation. Biochemical activities in different accession of pongamia pinnata l. Leaves of pongamia pinnata are digestive, laxative, antihelmintic and are good for diarrhea, leprosy. Virus free plant sub millimeter portion of shoottip stock plant virus free nodal cutting disadvantage of using small sized explant for micropropagation shoot tip explant low survival rate and slow initial growth. Micropropagation definition of micropropagation by the free. Micropropagation definition of micropropagation by the. Pongamia pinnata herb uses, benefits, cures, side effects. Pongamia pinnata pongam environmental horticulture university. Pongamia millettia pinnata syn ponamia pinnata summary pongamia millettia pinnata, formerly known as pongamia pinnata, is a treeshrub with a broad distribution from india, through central and southeastern asia, indonesia and into northern australia. The wood is yellowish white, coarse, hard, and beautifully grained, but not durable.
The method was validated for accuracy, precision, linearity. Morphological and molecular level of genetic diversity among. Our study was to screen the antibacterial activity of the seed extracts of pongamia pinnata linn. Department of botany, government vidarbha institute of science and humanities, amravati444604, india. Learn vocabulary, terms, and more with flashcards, games, and other study tools. The major defining benefit of using pongamia as a biofuel feedstock. An efficient protocol for micropropagation and genetic. Analysis of genetic diversity in pongamia pongamia pinnata l pierrre using aflp markers mahendar thudi 1, revathi manthena, suhas p wani, leela tatikonda 1,2, david a hoisington 1 and rajeev k varshney1,3, 1international crops research institute for the semiarid tropics, patancheru, greater hyderabad 502 324, andhra pradesh, india. The in vivo clonal propagation is often difficult, expensive and even unsuccessful. Clonal propagation of pongamia pinnata pierre through stem cuttings 103 clonal propagation of pongamia.
Reactiveextraction of pongamia seeds reactiveextraction of pongamia seeds was carried out using whole seeds with seed coat. A tissue culture technique for plant propagation in which tissue is taken from a plant and grown in a laboratory to produce plantlets that are. Nodulation in the legume biofuel feedstock tree pongamia pinnata. It is important to note that crude pongamia oil was used in this study for biodiesel preparation and since the degree of refinement of the vegetable oil affects the yield of ester formation freedman et al. This tree expands perfectly in areas having excellent daylight. Chemical examination of the seeds of pongamia pinnata l. Evaluation of antibacterial activity of pongamia pinnata. Micropropagation offers the potential to produce thousands or even millions of plants per annum.
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